Figure 5

A flow perfusion bioreactor with controlled mechanical stimulation: Application in cartilage tissue engineering and beyond

Bernard J Van Wie* and Arshan Nazempour

Published: 13 June, 2018 | Volume 2 - Issue 1 | Pages: 015-034

jsctt-aid1011-g005

Figure 5:

This diagram shows the overall experimental design. NC: negative control - base medium. PC: positive control (PC) – chondrogenic growth factor supplemented medium. AFM: atomic force microscopy. (a) A 10 μl droplet of expanded hADSCs at a density of 1.6×107 viable cells/ml was placed in the center of each of 15 wells in two 24-well culture plates. (b) 5×105 hADSCs in 500 μl of EM were centrifuged in each of two microcentrifuge tube sets at 600 g for 5 minutes, (c) 6×106 hADSCs in 2.5 ml of EM were injected into each reactor with two replicates at each condition, NC with and without oscillating hydrostatic pressure (OHP) and PC with and without OHP. (Figs. 5a&b reformatted with permission from a previous publication Copyright@American Scientific Publishers [105]; Fig. 5c reused in rearranged form with permission from Springer Nature: Springer-Verlag Berlin Heidelberg, Cell Tissue Res [109] © 2017).

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