Abstract

Research Article

Age-related changes in cell yield and viability of feline Adipose Tissue-Derived Mesenchymal Stem (fAD-MSCs)

Shazia Nissar*, Geetha Ramesh, Sabiha Hayath Basha, TA Kannan and Shahid Hussain

Published: 01 April, 2022 | Volume 6 - Issue 1 | Pages: 001-004

In the present study, omental adipose tissue was collected from, the animals that underwent ovariectomy and ovariohysterectomy, surgical procedures, at the age of seven months to 11/2 years of age groups. The sample was digested with 0.1% (W/V) collagenase type I and transferred to a beaker with a magnetic stirrer and kept in a stirrer with 600 rpm at 37 °C for 30 minutes. The viability of the cell was evaluated by the trypan blue exclusion method using a hemocytometer. Trypan blue had a high affinity to nuclear DNA, which traverse the member in a dead cell and dye it blue. In the present study, the cell yield of fAD-MSCs was 8.15 ± 0.68, 6.55 ± 0.26, 4.85 ± 0.42, 3.90 ± 0.34, and 3.51 ± 0.43 in different age groups viz., 7,8,9 month 1 and 1½ year respectively. In younger age groups, cell yield and viability percentage were more than in animals of higher age groups. In the younger age group, stem cells proliferation status is considered potent for therapeutic application.

Read Full Article HTML DOI: 10.29328/journal.jsctt.1001024 Cite this Article Read Full Article PDF

Keywords:

Adipose tissue; Cell viability; Cell yield; Feline; Mesenchymal; Omental; Stem cell; Regenerative medicine

References

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