Mesenchymal stem cells (MSCs) conditioned medium (CM) has a promising prospect towards skin regeneration. Therefore, human dental pulp and adipose stem cells (DPSCs and ADSCs) were isolated, propagated and evaluated for their stemness and genetic stability over time in culture before making CM. We aimed to characterize the applicability of lyophilized ADSCs and DPSCs derived CM (AD-CM and DP-CM) at 5 mg, 10 mg and 20 mg for wound healing process. The ability of wound closure was assessed by direct human dermal fibroblast cell scratch assay, treated with variable concentrations of AD-CM and DP-CM in vitro. Additionally, we also assessed the expression of different cytokines and growth factors secreted from ADSCs and DPSCs in the CM relevant to the wound healing by cytokine array analysis. Our data demonstrates a significant effect of both the AD-CM and DP-CM in wound healing within 24 hrs compared to that in control.
Su L, Zheng J, Wang Y, Zhang W, Hu D. Emerging progress on the mechanism and technology in wound repair. Biomed Pharmacother.2019;117:109191.PubMed: https://www.ncbi.nlm.nih.gov/pubmed/31387187
Jin Q, Yuan K, Lin W, Niu C, Ma R. Comparative characterization of mesenchymal stem cells from human dental pulp and adipose tissue for bone regeneration potential. Artif Cells Nanomed Biotechnol.2019;47:1577-1584. PubMed: https://www.ncbi.nlm.nih.gov/pubmed/31027424
Marei MK, El Backly RM. Dental Mesenchymal Stem Cell-Based Translational Regenerative Dentistry: From Artificial to Biological Replacement. Front Bioeng Biotechnol. 2018;6:49. PubMed: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5941981/
Shingyochi Y, Orbay H, Mizuno H. Adipose-derived stem cells for wound repair and regeneration. Expert Opin Biol Ther. 2015;15:1285-1292. PubMed: https://www.ncbi.nlm.nih.gov/pubmed/26037027
Hocking AM, Gibran NS. 2010. Mesenchymal stem cells: paracrine signaling and differentiation during cutaneous wound repair. Exp Cell Res. 2010;316:2213-2219.PubMed: https://www.ncbi.nlm.nih.gov/pubmed/20471978
Jayaraman P, Nathan P, Vasanthan P, Musa S, Govindasamy V. Stem cells conditioned medium: a new approach to skin wound healing management. Cell Biol Int. 2013;37:1122-1128. PubMed: https://www.ncbi.nlm.nih.gov/pubmed/23716460
Sagaradze G, Grigorieva O, Nimiritsky P, Basalova N, Kalinina N, et al. Conditioned Medium from Human Mesenchymal Stromal Cells: Towards the Clinical Translation. Int J Mol Sci. 2019;20.PubMed: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6479925/
Liang CC, Park AY, Guan JL. in vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nat Protoc. 2007;2:329-333. PubMed: https://www.ncbi.nlm.nih.gov/pubmed/17406593
Vizoso FJ, Eiro N, Cid S, Schneider J, Perez-Fernandez R. Mesenchymal Stem Cell Secretome: Toward Cell-Free Therapeutic Strategies in Regenerative Medicine. Int J Mol Sci. 2017;18. PubMed: https://www.ncbi.nlm.nih.gov/pubmed/28841158
Kim JH, Jung M, Kim HS, Kim YM, Choi EH. Adipose-derived stem cells as a new therapeutic modality for ageing skin. Exp Dermatol. 2011;20:383-387.PubMed: https://www.ncbi.nlm.nih.gov/pubmed/21355887
Mendicino M, Bailey AM, Wonnacott K, Puri RK, Bauer SR. MSC-based product characterization for clinical trials: an FDA perspective. Cell Stem Cell. 2014;14:141-145. PubMed: https://www.ncbi.nlm.nih.gov/pubmed/24506881
Park JW, Hwang SR, Yoon IS. Advanced Growth Factor Delivery Systems in Wound Management and Skin Regeneration. Molecules. 2017;27;22. PubMed: https://www.ncbi.nlm.nih.gov/pubmed/28749427
Simone TM, Longmate WM, Law BK, Higgins PJ. Targeted Inhibition of PAI-1 Activity Impairs Epithelial Migration and Wound Closure Following Cutaneous Injury. Adv Wound Care (New Rochelle). 2015;1;4:321-328. PubMed: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4440983/
Tello-Montoliu A, Patel JV, Lip GY. Angiogenin: a review of the pathophysiology and potential clinical applications. J Thromb Haemost. 2006;4:1864-1874. PubMed: https://www.ncbi.nlm.nih.gov/pubmed/16961595
Werner S, Grose R. Regulation of wound healing by growth factors and cytokines. Physiol Rev. 2003;83:835-870. PubMed: https://www.ncbi.nlm.nih.gov/pubmed/12843410
Philippe Hernigou*, Yasuhiro Homma, Arnaud Dubory, Jacques Pariat, Damien Potage, Charles Henri Flouzat Lachaniette, Nathalie Chevallier and Helene Rouard Philippe Hernigou*,Yasuhiro Homma,Arnaud Dubory,Jacques Pariat,Damien Potage,Charles Henri Flouzat Lachaniette,Nathalie Chevallier,Helene Rouard . The Femoral Head of Patients with Hip Dysplasia is not as Osteogenic as Iliac Crest Bone Location. . 2017 doi: 10.29328/journal.jsctt.1001001; 1: 001-007
Giuseppe Angelico*, Eliana Piombino, Giuseppe Broggi, Fabio Motta and Saveria SpadolaGiuseppe Angelico*,Eliana Piombino,Giuseppe Broggi,Fabio Motta,Saveria Spadola. Rhabdomyoblasts in Pediatric Tumors: A Review with Emphasis on their Diagnostic Utility . . 2017 doi: 10.29328/journal.jsctt.1001002; 1: 008-016
Benetti Federico*, Natalia Scialacomo, Enrique Mariani, Luis Geffner, Bruno Benetti Eng1, Daniel Brusich and Yan DuarteBenetti Federico*,Natalia Scialacomo,Enrique Mariani,Luis Geffner,Bruno Benetti Eng1,Daniel Brusich,Yan Duarte. Surgical Implantation of Stem Cells in Heart Failure Patients due to Idiophatic Cardiomyopathy. . 2017 doi: 10.29328/journal.jsctt.1001003; 1: 017-027
Bernard J Van Wie*, Arshan Nazempour##, Chrystal R Quisenberry## and Nehal I Abu-LailBernard J Van Wie*,Arshan Nazempour##,Chrystal R Quisenberry##,Nehal I Abu-Lail. Enhancing adipose stem cell chondrogenesis: A study on the roles of dexamethasone, transforming growth factor β3 and ascorbate supplements and their combination. . 2017 doi: 10.29328/journal.jsctt.1001004; 1: 028-051
Christine Skerka*, Yuchen Lin and Peter F. ZipfelChristine Skerka*,Yuchen Lin,Peter F. Zipfel. Nicotinamide as a treatment option of Age-Related Macular Degeneration. . 2017 doi: 10.29328/journal.jsctt.1001006; 1: 063-065
Seyed Hamed Mousavi*, Fateme Khorramroo and Hooman MinoonejadSeyed Hamed Mousavi*,Fateme Khorramroo,Hooman Minoonejad. Symmetry of Shank Muscle Strength, Passive Stiffness and Plantar Pressure Following IASTM Accompanied by Electrotherapy in a Case with Severe Ankle Stiffness. J Sports Med Ther. 2024: doi: 10.29328/journal.jsmt.1001089; 9: 091-095
Julian A Purrinos* and Ramzi YounisJulian A Purrinos*, Ramzi Younis. Sinonasal Myxoma Extending into the Orbit in a 4-Year Old: A Case Presentation. Arch Case Rep. 2024: doi: 10.29328/journal.acr.1001099; 8: 075-077
H Pérez-Aguilar*, M Lacruz-Asaro and F Arán-AisH Pérez-Aguilar*, M Lacruz-Asaro, F Arán-Ais. Evaluation of Biostimulants Based on Recovered Protein Hydrolysates from Animal By-products as Plant Growth Enhancers. J Plant Sci Phytopathol. 2023 doi: 10.29328/journal.jpsp.1001104; 7: 042-047
Denis Tonini, Kai Wu, Renata Saha and Jian-Ping Wang*Denis Tonini,Kai Wu,Renata Saha,Jian-Ping Wang*. Feasibility study of magnetic sensing for detecting single-neuron action potentials. Ann Biomed Sci Eng. 2022 doi: 10.29328/journal.abse.1001018; 6: 019-029
Julian A Purrinos* and Ramzi YounisJulian A Purrinos*, Ramzi Younis. Sinonasal Myxoma Extending into the Orbit in a 4-Year Old: A Case Presentation. Arch Case Rep. 2024 doi: 10.29328/journal.acr.1001099; 8: 075-077
Khashayar Maroufi*Khashayar Maroufi*. Physical activity can change the physiological and psychological circumstances during COVID-19 pandemic: A narrative review. J Sports Med Ther. 2021 doi: 10.29328/journal.jsmt.1001051; 6: 001-007
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