To repair articular cartilage (AC) defects in osteoarthritic patients, one approach is to engineer three-dimensional grafts with physicochemical properties similar to endogenous AC. Such grafts can be grown in bioreactors that provide environmental conditions favoring chondrogenesis. Studies show mechanical stimulation during the culturing process greatly enhances development of functional engineered grafts. A review of literature on bioreactor options reveals a lack of capacity to simultaneously stimulate cells with a combination of shear stress and oscillating hydrostatic pressure, both of which are important parts of the in vivo AC environment. It is hypothesized that combining both forces in a new bioreactor design will contribute to better AC tissue growth. In this paper, we provide a brief review of bioreactors and describe a new computer-controlled perfusion and pressurized bioreactor system, and the novelty of its control programming features for service in a host of applications. We briefly summarize results on synergistic effects in employing perfusion, oscillating hydrostatic pressure in a scaffold free environment and with the addition of encapsulation for inducing chondrogenesis. We further describe efforts to modify the newly developed system to include a continuous flow and pressurized centrifugal mode to enhance further the capabilities for inclusion of very high shear stresses. Applications for several other cell and tissue engineering approaches are discussed.
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Kesti M, Müller M, Becher J, Schnabelrauch M, D'Este M. A versatile bioink for three-dimensional printing of cellular scaffolds based on thermally and photo-triggered tandem gelation. Acta Biomater. 2015; 11: 162-172. Ref.: https://tinyurl.com/y7zlq4y6
Das S, Pati F, Choi YJ, Rijal G, Shim JH, et al. Bioprintable, cell-laden silk fibroin-gelatin hydrogel supporting multilineage differentiation of stem cells for fabrication of three-dimensional tissue constructs. Acta Biomater. 2015; 11: 233-246. Ref.: https://tinyurl.com/y95kcppj
Shoichet MS, Li RH, White ML, Winn SR. Stability of hydrogels used in cell encapsulation: An in vitro comparison of alginate and agarose. Biotechnol Bioeng. 1996; 50: 374-381. Ref.: https://tinyurl.com/yd8q3ltf
Smith CM, Stone AL, Parkhill RL, Stewart RL, Simpkins MW, et al. Three-dimensional bioassembly tool for generating viable tissue-engineered constructs. Tissue Eng. 2004; 10: 1566-76. Ref.: https://tinyurl.com/y9dlduvu
Nazempour A, Van Wie BJ. Chondrocytes, mesenchymal stem sells, and their combination in articular cartilage regenerative medicine. Annals of Biomedical Engineering. 2016; 44: 1325-1354. Ref.: https://tinyurl.com/ydyjukdj
Scaglione S, Wendt D, Miggino S, Papadimitropoulos A, Fato M, et al. Effects of fluid flow and calcium phosphate coating on human bone marrow stromal cells cultured in a defined 2D model system. J Biomed Mater Res A. 2008; 86: 411-419. Ref.: https://tinyurl.com/ybdqy8h7
Kreke MR, Huckle WR, Goldstein AS. Goldstein. Fluid flow stimulates expression of osteopontin and bone sialoprotein by bone marrow stromal cells in a temporally dependent manner. Bone, 2005; 36: 1047-1055. Ref.: https://tinyurl.com/ybe9rl7q
Kapur S, Baylink DJ, Lau KH. Fluid flow shear stress stimulates human osteoblast proliferation and differentiation through multiple interacting and competing signal transduction pathways. Bone. 2003; 32: 241-251. Ref.: https://tinyurl.com/ydyo7ses
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Ecker DM, Jones SD, Levine HL. The therapeutic monoclonal antibody market. MAbs. 2015; 7: 9-14. Ref.: https://tinyurl.com/ycdchsan
Detzel CJ, Mason DJ, Davis WC, van Wie BJ. Kinetic simulation of a centrifugal bioreactor for high population density hybridoma culture. Biotechnol Prog. 2009; 25: 1650-1659.
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Bernard J Van Wie* and Arshan NazempourBernard J Van Wie*,Arshan Nazempour. A flow perfusion bioreactor with controlled mechanical stimulation: Application in cartilage tissue engineering and beyond. . 2018 doi: 10.29328/journal.jsctt.1001011; 2: 015-034
Hessam Nowzari*, Mao-Chi Tuan, Michael Jorgensen, Marie-Grace Michel and Jean-Francois MichelHessam Nowzari*,Mao-Chi Tuan,Michael Jorgensen,Marie-Grace Michel,Jean-Francois Michel. Dead sea salt solution: composition, lack of cytotoxicity and in vitro efficacy against oral leukotoxins, endotoxins and glucan sucrose. Insights Biol Med. 2022: doi: 10.29328/journal.ibm.1001021; 6: 009-014
María Elena Trujillo Ortega, Selene Fernández Hernández, Montserrat Elemi García Hernández, Rolando Beltrán Figueroa, Francisco Martínez Castañeda, Claudia Itzel Vergara Zermeño, Sofía Lizeth Alcaráz Estrada, Elein Hernández Trujillo and Rosa Elena Sarmiento Silva*María Elena Trujillo Ortega,Selene Fernández Hernández,Montserrat Elemi García Hernández,Rolando Beltrán Figueroa,Francisco Martínez Castañeda,Claudia Itzel Vergara Zermeño,Sofía Lizeth Alcaráz Estrada,Elein Hernández Trujillo,Rosa Elena Sarmiento Silva*. Comparing Immunity Elicited by Feedback and Titered Viral Inoculation against PEDV in Swine. Insights Vet Sci. 2024: doi: 10.29328/journal.ivs.1001044; 8: 028-038
Gideon S Alex*, Olanrewaju Oluwaseun Oke, Joy Wilberforce Ekokojide, Tolulope Judah Gbayisomore, Anene-Ogbe C Martina, Farounbi Glory and Joshua Ayodele YusufGideon S Alex*,Olanrewaju Oluwaseun Oke,Joy Wilberforce Ekokojide,Tolulope Judah Gbayisomore,Anene-Ogbe C Martina,Farounbi Glory,Joshua Ayodele Yusuf. Adult Neurogenesis: A Review of Current Perspectives and Implications for Neuroscience Research. J Neurosci Neurol Disord. 2024: doi: 10.29328/journal.jnnd.1001102; 8: 106-114
H Pérez-Aguilar*, M Lacruz-Asaro and F Arán-AisH Pérez-Aguilar*, M Lacruz-Asaro, F Arán-Ais. Evaluation of Biostimulants Based on Recovered Protein Hydrolysates from Animal By-products as Plant Growth Enhancers. J Plant Sci Phytopathol. 2023 doi: 10.29328/journal.jpsp.1001104; 7: 042-047
Denis Tonini, Kai Wu, Renata Saha and Jian-Ping Wang*Denis Tonini,Kai Wu,Renata Saha,Jian-Ping Wang*. Feasibility study of magnetic sensing for detecting single-neuron action potentials. Ann Biomed Sci Eng. 2022 doi: 10.29328/journal.abse.1001018; 6: 019-029
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