Abstract

Research Article

Intraepidermal Injections of Autologous Epidermal Cell Suspension: A new promising approach to Dermatological Disorders. Preliminary Study

Fabio Rinaldi*, Elisa Borsani, Luigi Fabrizio Rodella, Elisabetta Sorbellini, Rita Rezzani, Daniela Pinto, Barbara Marzani, Giovanna Tabellini and Mariangela Rucco

Published: 07 December, 2017 | Volume 1 - Issue 1 | Pages: 066-070

Regenerative medicine is a modern approach of dermatological treatment, using Epidermal Cells of the interfollicular epidermis (ESCs) for their effect in skin regeneration in chronic ulcers and burns, melanoma, vitiligo, junctional epidermolysis bullosa. Intraepidermal injections of autologous epidermal cell suspension can be a new and very promising treatment for many other cutaneous disorders as non-scarring alopecia (Alopecia Areata, Androgenic Alopecia) or scarring alopecia (Lichern Plano Pilaris alopecia, Discoid Lupus Erithematosus alopecia), anti-aging therapies. The intraepidermal injection of an autologous epidermal cell suspension is a simple, fast and safe surgical procedure: a small, thin portion of the epidermis of the patient undergoes a treatment where a suspension with all the cells collected from the epidermis and cultured for 7 days is injected into the skin. Our preliminary study shows that a suspension contains a significant number of viable cells that survive at day 7 in culture.

Our research is ongoing and is focusing on the typing of the different cells in the suspension and evaluation of the presence and the nature of stem cells.

Read Full Article HTML DOI: 10.29328/journal.jsctt.1001007 Cite this Article Read Full Article PDF

References

  1. Jackson CJ, Tønseth KA, Utheim TP. Cultured epidermal stem cells in regenerative medicine. Stem Cell Res Ther. 2017; 8: 155. Ref: https://goo.gl/x7nfCo
  2. University of Chicago. Chemotherapy plus peripheral stem cell transplantation in treating patients with melanoma or metastatic kidney cancer. ClinicalTrials.gov. 2014; NCT00004135.
  3. Hoerter JD, Bradley P, Casillas A, Chambers D, Weiswasser B, et al. Does melanoma begin in a melanocyte stem cell? J Skin Cancer. 2012. Ref: https://goo.gl/hxe7af
  4. Ann & Robert H Lurie Children’s Hospital of Chicago. Immunoablative mini transplant (hematopoietic peripheral blood stem cell transplant [HPBSC]). ClinicalTrials.gov. 2014; NCT00179764. Ref: https://goo.gl/xzTavg
  5. Fred Hutchinson Cancer Research Center. Combination chemotherapy, total-body irradiation, peripheral stem cell transplantation, and lymphocyte infusion in treating patients with Stage IV melanoma. ClinicalTrials.gov 2011; NCT00006233. Ref: https://goo.gl/RAzGH2
  6. Khodadadi L, Shafieyan S, Sotoudeh M, Dizaj AV, Shahverdi A, et al. Intraepidermal injection of dissociated epidermal cell suspension improves vitiligo. Arch Dermatol Res. 2010; 302: 593-599. Ref: https://goo.gl/PCCV95
  7. Van Geel N, Ongenae K, Naeyaert JM. Surgical techniques for vitiligo: a review. Dermatology. 2001; 202: 162-166. Ref: https://goo.gl/QJCLm9
  8. Lee DY, Lee JH. Epidermal grafting for vitiligo: a comparison of cultured and noncultured grafts. Clin Exp Dermatol. 2010; 35: 325-326. Ref: https://goo.gl/WMpBw6
  9. Guerra L, Capurro S, Melchi F, Primavera G, Bondanza S, et al. Treatment of "stable" vitiligo by Timedsurgery and transplantation of cultured epidermal autografts. Arch Dermatol. 2000; 136: 1380-1389. Ref: https://goo.gl/BZqtQk
  10. Matsuzaki K, Kumagai N. Treatment of vitiligo with autologous cultured keratinocytes in 27 cases. Eur J Plast Surg. 2013; 36: 651-656. Ref: https://goo.gl/SWQegA
  11. Mavilio F, Pellegrini G, Ferrari S, Di Nunzio F, Di Iorio E, et al. Correction of junctional epidermolysis bullosa by transplantation of genetically modified epidermal stem cells. Nat Med. 2006; 12: 1397-1402. Ref: https://goo.gl/3rabfK
  12. Trink A, Sorbellini E, Bezzola P, Rodella L, Rezzani R, et al. A randomized, double-blind, placebo- and active-controlled, half-head study to evaluate the effects of platelet-rich plasma on alopecia areata. Br J Dermatol. 2013; 169: 690-694. Ref: https://goo.gl/8uhusp
  13. Allen TD, Potten CS. Fine-structural identification and organization of the epidermal proliferative unit. J Cell Sci. 1974; 15: 291-319. Ref: https://goo.gl/G1e472
  14. Ghazizadeh S, Taichman LB. Multiple classes of stem cells in cutaneous epithelium: a lineage analysis of adult mouse skin. The EMBO Journal. 2001; 20: 1215-1222. Ref: https://goo.gl/t4wVRJ
  15. Purba TS, Haslam IS, Poblet E, Jiménez F, Gandarillas A, et al. Human epithelial hair follicle stem cells and their progeny: current state of knowledge, the widening gap in translational research and future challenges. Bioassays. 2014; 36: 513-525. Ref: https://goo.gl/4Vte5v
  16. Gottipamula S, Saraswat SK, Sridhar KN. Comparative study of isolation, expansion and characterization of epithelial cells. Cytotherapy. 2017; 19: 263-271. Ref: https://goo.gl/CYY5kc
  17. De Kock J, Rodrigues RM, Buyl K, Vanhaecke T, Rogiers V. Human Skin-Derived Precursor Cells: Isolation, Expansion, and Hepatic Differentiation. Methods Mol Biol. 2015; 1250: 113-122. Ref: https://goo.gl/9qhxvg
  18. Fujimori Y, Izumi K, Feinberg SE, Marcelo CL. Isolation of small-sized human epidermal progenitor/stem cells by Gravity Assisted Cell Sorting (GACS). J Dermatol Sci. 2009; 56: 181-187. Ref: https://goo.gl/cAawnP
  19. Kaviani M, Geramizadeh B, Rahsaz M, Marzban S. Considerations in the improvement of human epidermal keratinocyte culture in vitro. Exp Clin Transplant. 2015; 13: 366-370. Ref: https://goo.gl/BY7Gsu
  20. Li A, Simmons PJ, Kaur P. Identification and isolation of candidate human keratinocyte stem cells based on cell surface phenotype. Proc Natl Acad Sci USA. 1998; 95: 3902-3907. Ref: https://goo.gl/A6EEhY

Figures:

Figure 1

Figure 1

Figure 1

Figure 2

Figure 1

Figure 3

Similar Articles

Recently Viewed

Read More

Most Viewed

Read More

Help ?